DOT1L antibody (AA 1000-1050)

Catalog No. ABIN1978542

The Rabbit Polyclonal anti-DOT1L antibody (ABIN1978542) specifically detects DOT1L in WB, IHC, IHC (p), IP and IF. The antibody is reactive with Human samples.

Quick Overview for DOT1L antibody (AA 1000-1050) (ABIN1978542)

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOT1L antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-DOT1L Antibody

Binding Specificity: AA 1000-1050

Specificity: Region between residue1000 and 1050 of human DOT1-Like using the numbering given in entry NP_115871.1 (GeneID 84444).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:50 - 1:500), IHC, IHC-P (1:200 - 1:1000), IP (2 - 5 μg/mg lysate), WB (1:2000 - 1:10000)

Usage: Immunohistochemistry: Antigen retrieval with Tris-EDTA  pH 9.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Colon Carcinoma, Lung Adenocarcinoma, Ovarian Carcinoma, Prostate Carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline, 0.1 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for DOT1L

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Alternative Name: DOT1L / DOT1

Background: Name/Gene ID: DOT1L

Synonyms: DOT1L, DOT1, DOT1-like protein, KMT4, Lysine N-methyltransferase 4, KIAA1814, H3-K79-HMTase

Gene ID: 84444