DOT1L antibody (C-Term)

Catalog No. ABIN1978544

The Rabbit Polyclonal anti-DOT1L antibody has been validated for IHC, IHC (p), IP and IF. It is suitable to detect DOT1L in samples from Human, Mouse and Rat.

Quick Overview for DOT1L antibody (C-Term) (ABIN1978544)

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOT1L antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-DOT1L Antibody

Binding Specificity: C-Term

Specificity: Region between residue1485 and the C-terminus (residue 1537) of human DOT1-Like using the numbering given in entry NP_115871.1 (GeneID 84444).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:250 - 1:2000), IHC, IHC-P (1:500 - 1:2000), IP (2 - 5 μg/mg lysate)

Usage: Immunohistochemistry: Antigen retrieval with Tris-EDTA  pH 9.0 is recommended for FFPE tissue sections.

Not recommended for: WB

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for DOT1L

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Alternative Name: DOT1L / DOT1

Background: Name/Gene ID: DOT1L

Synonyms: DOT1L, DOT1, DOT1-like protein, KMT4, Lysine N-methyltransferase 4, KIAA1814, H3-K79-HMTase

Gene ID: 84444