E2F1 antibody (AA 300-350)

Catalog No. ABIN1978600

This Rabbit Polyclonal antibody specifically detects E2F1 in IHC, IP, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for E2F1 antibody (AA 300-350) (ABIN1978600)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-E2F1 Antibody

Binding Specificity: AA 300-350

Specificity: Region between residue 300 and 350 of human E2F Transcription Factor 1 using the numbering given in entry NP_005216.1 (GeneID 1869).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:50 - 1:500), IHC, IHC-P (1:500 - 1:2000), IP (2 - 5 μg/mg lysate)

Usage: Immunohistochemistry: Antigen retrieval with Tris-EDTA  pH 9.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma.

Not recommended for: WB

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background: Name/Gene ID: E2F1

Synonyms: E2F1, E2F transcription factor 1, PRB-binding protein E2F-1, RBAP-1, RBBP3, RBBP-3, Transcription factor E2F1, RBAP1, E2F-1, PBR3

Gene ID: 1869

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy