TLR4 antibody (Biotin)

Catalog No. ABIN198147

This anti-TLR4 antibody is a Mouse Monoclonal antibody detecting TLR4 in FACS, ELISA, IP and Func. Suitable for Human.

Quick Overview for TLR4 antibody (Biotin) (ABIN198147)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR4 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Functional Studies (Func)

Product Details anti-TLR4 Antibody (Biotin)

Specificity: Recognizes human TLR4 (Toll Like Receptor 4) (Biotin).

Purification: Purified

Immunogen: Ba/F3 cell line expressing human TLR4 cell surface antigen.

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Flo, Func, IP

Usage: ELISA, Flow cytometry. Functional studies: It has been shown to block the activation of monocytes with LPS). Immunoprecipitation: suitable. Recommended Dilution: ELISA: 1 μg/mL. Flow cytometry: 2-5 μg/1 x 10 cells. However, the optimal condition has to be determined for individual experiments. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: TLR4

Background: Name/Gene ID: TLR4
Family: Toll-like Receptor

Synonyms: TLR4, CD284, CD284 antigen, Homolog of Drosophila toll, TLR-4, TOLL, ARMD10, Toll-like receptor 4, HToll

Gene ID: 7099

UniProt: O00206

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins