TLR4 antibody

Catalog No. ABIN198156

This anti-TLR4 antibody is a Rat Monoclonal antibody detecting TLR4 in FACS and IP. Suitable for Mouse.

Quick Overview for TLR4 antibody (ABIN198156)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-TLR4 Antibody

Specificity: Reacts preferentially, especially in flow cytometry, with mouse TLR4 that is associated with MD-2. Recognizes the mouse TLR4/MD-2 complex and is not useful for detection of TLR4 alone.

Purification: Purified

Immunogen:

Type of Immunogen: Native protein

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:10), IP

Usage: Can be used for Flow Cytometry and Immunoprecipitation. The antibody recognizes the mouse TLR4/MD-2 complex and is not useful for detection of TLR4 alone. Furthermore the antibody can be used as antagonist for TLR4, it blocks LPS-induced cytokine production. Flow Cytometry: 1:10. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide, 0.1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: TLR4

Background: Name/Gene ID: TLR4
Family: Toll-like Receptor

Synonyms: TLR4, CD284, CD284 antigen, Homolog of Drosophila toll, TLR-4, TOLL, ARMD10, Toll-like receptor 4, HToll

Gene ID: 7099

UniProt: O00206

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins