CCR7 antibody (PE)

Catalog No. ABIN1981861

This Rat Monoclonal antibody specifically detects CCR7 in FACS. It exhibits reactivity toward Mouse and has been mentioned in 3+ publications.

Quick Overview for CCR7 antibody (PE) (ABIN1981861)

Target: CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CCR7 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 4B12

Product Details anti-CCR7 Antibody (PE)

Purpose: Anti-Ms CD197 PE

Specificity: The rat monoclonal antibody 4B12 recognizes an extracellular epitope of murine CD197 / CCR7 (chemokine receptor 7), a 43 kDa G-protein-coupled receptor for chemokines CCL19 and CCL21.

Cross-Reactivity (Details): Mouse

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Murine CD197-transfected RBL-2H3 cells

Isotype: IgG2a kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CCR7 antibody (PE) (ABIN1981861)

Adachi, Osada, Nakamura, Tamada, Hamano: "Unique T cells with unconventional cytokine profiles induced in the livers of mice during Schistosoma mansoni infection." in: PLoS ONE, Vol. 8, Issue 12, pp. e82698, (2013) (PubMed).

Eisenbarth, Williams, Colegio, Meng, Strowig, Rongvaux, Henao-Mejia, Thaiss, Joly, Gonzalez, Xu, Zenewicz, Haberman, Elinav, Kleinstein, Sutterwala, Flavell: "NLRP10 is a NOD-like receptor essential to initiate adaptive immunity by dendritic cells." in: Nature, Vol. 484, Issue 7395, pp. 510-3, (2012) (PubMed).

Huggins, Paschalidis, Flower, Perretti, DAcquisto: "Annexin-1-deficient dendritic cells acquire a mature phenotype during differentiation." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 23, Issue 4, pp. 985-96, (2009) (PubMed).

Target Details for CCR7

Target: CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))

Alternative Name: CD197

Background: Chemokine (C-C motif) receptor 7,CD197 / CCR7 (chemokine receptor 7) is a seven membrane-spanning protein serving as a receptor for chemokines CCL19 and CCL21. It is expressed on most naive T cells, some hematopoietic stem cells, mature dendritic cells, NK cells and some memory T cells and B cells subsets. CD197 plays important roles in development of thymocytes, and regulating the recirculation and homing of lymphocytes and dendritic cells to secondary lymphoid organs.,CCR7, CC-CKR-7, EBI1

Gene ID: 12775

UniProt: P47774

Pathways: Regulation of Actin Filament Polymerization, Positive Regulation of Immune Effector Process