CCR7 antibody (Extracellular, N-Term)

Catalog No. ABIN7886249

This Rabbit Polyclonal antibody specifically detects CCR7 in WB, FACS, IHC and LCI. It exhibits reactivity toward Human.

Quick Overview for CCR7 antibody (Extracellular, N-Term) (ABIN7886249)

Target: CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCR7 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Product Details anti-CCR7 Antibody

Binding Specificity: AA 26-40, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to CCR7 Chemokine Receptor

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,mouse - 14,15 amino acid residues identical

Characteristics: Anti-CCR7 (extracellular) Antibody (ABIN7043035 and ABIN7044176) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize CCR7 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DEVTDDYIGDNTTVD, corresponding to amino acid residues 26-40 of human CCR7

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235055)

Blocking Peptide: (ABIN7235055)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CCR7

Target: CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))

Alternative Name: CCR7

Background:

Synonyms: C-C chemokine receptor type 7, C-C CKR-7, Epstein-Barr virus-induced G-protein coupled receptor 1, EBI1, CD197

Description: Chemokines (CHEMOtactic cytoKINES) are an important subgroup of the inflammatory cytokine family. More than fifty chemokines are expressed in mammalian cells and are characterized by their relatively small size (~70-90 amino acids), by their conserved N-terminus and cysteine motifs. This group of proteins has been further categorized on the basis of the cysteine spacing in the motifs creating C, CC, CXC, and CX3C chemokine subfamilies1,2.All fifty chemokines exert their effects through twenty different chemokine receptors, belonging to the superfamily of G-protein coupled receptors (GPCRs) suggesting a certain level of promiscuity among the different receptors. All chemokine receptors couple to the pertussis sensitive Gi protein leading to phospholipase C activation and adenylate cyclase inhibition3.Chemokines were first identified by their ability to mediate leukocyte chemoattraction. Apart from regulating the migration of leukocytes, they seem to be major players during inflammation and immunity4-6. Indeed, chemokines could also be further classified as being inflammatory as many chemokines are extensively upregulated in response to inflammation, or housekeeping important for the homeostasis of certain cell types. Inflammatory chemokines are responsible for recruiting immune cells to the inflamed region, and housekeeping chemokines, expressed in lymphoid or non-lymphoid tissues mediate the trafficking and targeting of cells7,8.In general, chemokines and their receptors guide leukocytes to sites of infection/inflammation. However, cases of chronic inflammatory disease and tissue damage occur when there is excessive recruitment of leukocytes. They could also be involved in the pathogenesis of neurological diseases like multiple sclerosis and many inflammatory diseases like atherosclerosis and inflammatory bowel disease. Recently, chemokines and their receptors have been found to be involved in cancer metastasis, namely breast cancer1. The chemokine signaling also seems to be important for the communication between neural cells and the immune system, especially in the context of infection.CCR7 receptor is responsible for directing dendritic cell migration to the lymph nodes where they are important for initiating the immune response. This receptor has two ligands, CCL19 and CCL21 which are expressed by stromal cells in the T cell-rich lymph node area9-11.

Gene ID: 1236

NCBI Accession: NM_001301714

UniProt: P32248

Pathways: Regulation of Actin Filament Polymerization, Positive Regulation of Immune Effector Process