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Perforin 1 antibody

This Mouse Monoclonal antibody specifically detects Perforin 1 in IP, FACS, ICC, IHC (fro) and IHC (p). It exhibits reactivity toward Human and Cow and has been mentioned in 4+ publications.
Catalog No. ABIN1981903

Quick Overview for Perforin 1 antibody (ABIN1981903)

Target

See all Perforin 1 (PRF1) Antibodies
Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reactivity

  • 116
  • 29
  • 8
  • 7
  • 6
  • 5
  • 2
  • 1
  • 1
  • 1
Human, Cow

Host

  • 75
  • 65
  • 3
Mouse

Clonality

  • 77
  • 66
Monoclonal

Conjugate

  • 70
  • 17
  • 15
  • 5
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Perforin 1 antibody is un-conjugated

Application

  • 58
  • 57
  • 39
  • 22
  • 21
  • 18
  • 10
  • 9
  • 7
  • 6
  • 5
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

DG9
  • Purpose

    Anti-Perforin Purified

    Specificity

    The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.

    No Cross-Reactivity

    Mouse

    Cross-Reactivity (Details)

    Human, Bovine

    Purification

    Purified by protein-A affinity chromatography.

    Purity

    > 95 % (by SDS-PAGE)

    Immunogen

    purified granules from human YT lymphoma cell line

    Isotype

    IgG2b kappa
  • Application Notes

    Flow cytometry: Recommended dilution: 3-12 μg/mL. Intracellular staining.

    Restrictions

    For Research Use only
  • Concentration

    1 mg/mL

    Buffer

    Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Do not freeze. Do not use after expiration date stamped on vial label.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C. Do not freeze.
  • Li, Jeong, Song: "Molecular Characteristics and Distribution of Adult Human Corneal Immune Cell Types." in: , Vol. 13, pp. 798346, (2022) (PubMed).

    Qiu, Chen, Liao, Zhang, Wang, Li, Luo, Fang, Li, Zhou, Shen, Chen, Huang, Cai, Cao, Jiang, Zeng, Chen: "Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions." in: PLoS pathogens, Vol. 8, Issue 11, pp. e1002984, (2012) (PubMed).

    Takeuchi, Inoue, Otani, Yamasaki, Nakamura, Kibata: "Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT." in: Journal of molecular cell biology, Vol. 2, Issue 3, pp. 139-51, (2010) (PubMed).

    Hersperger, Makedonas, Betts: "Flow cytometric detection of perforin upregulation in human CD8 T cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 73, Issue 11, pp. 1050-7, (2008) (PubMed).

  • Target

    Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

    Alternative Name

    Perforin

    Background

    Perforin 1,Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.,PRF1, P1, PFP, HPLH2

    Gene ID

    5551

    UniProt

    P14222

    Pathways

    Apoptosis, Caspase Cascade in Apoptosis
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