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CD11b antibody (Biotin)

ITGAM Reactivity: Human, Rat FACS, IHC, IHC (fro) Host: Mouse Monoclonal Biotin

Catalog No. ABIN198550

Datasheet as PDF

Target See all CD11b (ITGAM) Antibodies

CD11b (ITGAM) (Integrin alpha M (ITGAM))
Reactivity
252

166

65

34

20

19

17

14

11

10

7

4

4

2

2

1
Human, Rat
Host
138

105

105

2

1

1
Mouse
Compatible Secondaries
Clonality
254

94

2
Monoclonal
Conjugate
119

39

29

27

20

9

7

7

5

5

5

5

4

4

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
This CD11b antibody is conjugated to Biotin
Application
264

82

80

78

60

44

36

24

23

19

14

14

7

6

5

5

4

4

3

2

2

1

1

1
Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity

Recognizes rat CD11B, the receptor for the iC3b component of complement. It is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and ~35 % of alveolar macrophages. This antibody also labels dendritic cells, granulocytes and microglial cells in the brain.

Purification

Protein G purified

Immunogen

Rat peritoneal macrophages.

Type of Immunogen: Cells

Isotype

IgG2a

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ITGAM Reactivity: Human, Mouse, Rat FACS Host: Rabbit Polyclonal unconjugated

anti-Integrin alpha M (ITGAM) (AA 253-282), (N-Term) antibody
ITGAM Reactivity: Human FACS, WB, IHC (p) Host: Rabbit Polyclonal RB17991 unconjugated

anti-Integrin alpha M (ITGAM) (AA 145-340) antibody
ITGAM Reactivity: Human IHC, WB, IF Host: Rabbit Polyclonal unconjugated

anti-Integrin alpha M (ITGAM) (AA 77-180) antibody
ITGAM Reactivity: Human, Mouse, Rat FACS, WB, IHC (fro), ELISA, IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-Integrin alpha M (ITGAM) (AA 31-160) antibody
ITGAM Reactivity: Rat IHC, WB, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Integrin alpha M (ITGAM) antibody (FITC)
ITGAM Reactivity: Human FACS Host: Mouse Monoclonal MEM-174 FITC

anti-Integrin alpha M (ITGAM) antibody (Biotin)
ITGAM Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-174 Biotin

anti-Integrin alpha M (ITGAM) antibody (PE)
ITGAM Reactivity: Human FACS Host: Mouse Monoclonal MEM-174 PE

anti-Integrin alpha M (ITGAM) (AA 623-728) antibody
ITGAM Reactivity: Human ELISA, ICC Host: Mouse Monoclonal 3A10H5 unconjugated

anti-Integrin alpha M (ITGAM) (AA 941-1074) antibody
ITGAM Reactivity: Human IHC, WB, StM Host: Mouse Monoclonal ITGAM-3340 unconjugated

Application Notes

Approved: Flo, IHC, IHC-Fr

Usage: Suitable for use in Flow Cytometry and Immunohistochemistry. Flow Cytometry: Neat-1:10, Use 10 μL to label 1 x10⁶ in 100 μL. Immunohistochemistry: Frozen. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment

Target Species of Antibody: Rat

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS,  pH 7.2, 0.1 % sodium azide, 1 % BSA.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
Target

CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name

ITGAM / CD11b (ITGAM Products)

Background

Name/Gene ID: ITGAM
Family: Integrin

Synonyms: ITGAM, Antigen CD11b (p170), CD11B, CD11b antigen, CR3A, Integrin alpha-M, MAC-1, MO1A, Neutrophil adherence receptor, MAC1A, SLEB6, CR-3 alpha chain

Gene ID

3684

UniProt

P11215

Pathways

Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation