CD11b antibody (Biotin)

Catalog No. ABIN198550

This Mouse Monoclonal antibody specifically detects CD11b in FACS, IHC and IHC (fro). It exhibits reactivity toward Human and Rat.

Quick Overview for CD11b antibody (Biotin) (ABIN198550)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD11b antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CD11b Antibody (Biotin)

Specificity: Recognizes rat CD11B, the receptor for the iC3b component of complement. It is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and ~35 % of alveolar macrophages. This antibody also labels dendritic cells, granulocytes and microglial cells in the brain.

Purification: Protein G purified

Immunogen: Rat peritoneal macrophages.

Type of Immunogen: Cells

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo, IHC, IHC-Fr

Usage: Suitable for use in Flow Cytometry and Immunohistochemistry. Flow Cytometry: Neat-1:10, Use 10 μL to label 1 x10⁶ in 100 μL. Immunohistochemistry: Frozen. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: ITGAM / CD11b

Background: Name/Gene ID: ITGAM
Family: Integrin

Synonyms: ITGAM, Antigen CD11b (p170), CD11B, CD11b antigen, CR3A, Integrin alpha-M, MAC-1, MO1A, Neutrophil adherence receptor, MAC1A, SLEB6, CR-3 alpha chain

Gene ID: 3684

UniProt: P11215

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation