Connexin 43/GJA1 antibody (AA 252-270)

Catalog No. ABIN199018

This Mouse Monoclonal antibody specifically detects Connexin 43/GJA1 in WB, IHC, ELISA, ICC and IHC (fro). It exhibits reactivity toward Human, Rat, Mouse, Dog and Pig.

Quick Overview for Connexin 43/GJA1 antibody (AA 252-270) (ABIN199018)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Human, Rat, Mouse, Dog, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Connexin 43/GJA1 Antibody

Binding Specificity: AA 252-270

Specificity: Recognizes mouse Connexin 43 (43-47kD). No known homology with other connexins. Species cross-reactivity: Canine, porcine, rat and human.

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to aa252-270 of the mouse connexin 43 sequence.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, ICC (1:50 - 1:250), IHC, IHC-Fr, WB (1:1000 - 1:10000)

Usage: Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and ELISA. Western Blot (colorimetric detection): 1:1000-1:10000 (Mouse brain lysates). Immunocytochemistry: 1:50-1:250. Immunohistochemistry: 1:50-1:250 (Frozen tissue sections of mouse keratinocytes and rat heart).

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: GJA1 / CX43 / Connexin 43

Background: Name/Gene ID: GJA1
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJA1, Connexin-43, DFNB38, Connexin 43, Gap junction alpha-1 protein, GJAL, HSS, Gap junction protein alpha 1, ODDD, ODD, AVSD3, CX43, HLHS1, ODOD, SDTY3

Gene ID: 2697

UniProt: P17302

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization