beta Amyloid antibody

Catalog No. ABIN1990945

This Mouse Monoclonal antibody specifically detects beta Amyloid in ELISA, DB and IL. It exhibits reactivity toward Human.

Quick Overview for beta Amyloid antibody (ABIN1990945)

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Amyloid antibody is un-conjugated

Application: ELISA, Dot Blot (DB), Immunolocalization (IL)

Clone: 3E5-F8

Product Details anti-beta Amyloid Antibody

Purpose: Anti-mAB-O | Mouse anti-human Abeta protein (3-10) region, oligomer-specific (clone 3E5,F8)

Cross-Reactivity (Details): Human
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Mouse,Bovine,Chicken,Dog,Porcine,Rabbit

Purification: Immunogen affinity purified serum

Immunogen: Synthetic peptide chosen from human Abeta (1-42) protein.

Isotype: IgG1, kappa

Application Details

Application Notes: 10 μg/mL (IL), 1-2 μg/mL (Dot), 1 μg/mL (ELISA capture)

Comment:

Immunolocalization: human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95°C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for beta Amyloid

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Alternative Name: Abeta

Background: Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein. Recent findings however suggest that smaller oligomeric forms of Abeta, formed in parallel to the amyloid plaques, excert the predominant tissue damaging effect.Specific identification of the oligomeric forms is as a consequence of great interest. Based on a recently published technique a highly oligomer-specific antibody (mAB-O), targeting Abeta oligomers while omitting reactivity towards the monomeric and fibrillar counterpart, has been developed.

Molecular Weight: 4.5 kDa

Pathways: Inflammasome