E2F1 antibody

Catalog No. ABIN199117

This Mouse Monoclonal antibody specifically detects E2F1 in WB, IHC and IP. It exhibits reactivity toward Human.

Quick Overview for E2F1 antibody (ABIN199117)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-E2F1 Antibody

Specificity: Reacts specifically with human E2F-1 protein. It does not cross-react with other related E2F protein. Reactivity with this antibody has been confirmed for human cell lysates.

Purification: Protein A purified

Immunogen: Recombinant human E2F-1 protein.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: IHC (0.1 - 1 μg/mL), IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Immunohistochemistry: 0.1-1.0 μg/mL. Immunoprecipitation: 2-5 μg/IP. Western Blot: 1-2 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background: Name/Gene ID: E2F1

Synonyms: E2F1, E2F transcription factor 1, PRB-binding protein E2F-1, RBAP-1, RBBP3, RBBP-3, Transcription factor E2F1, RBAP1, E2F-1, PBR3

Gene ID: 1869

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy