GRIA2 antibody (AA 175-430)

Catalog No. ABIN199199

The Mouse Monoclonal anti-GRIA2 antibody has been validated for WB, IHC, ELISA, ICC and RIA. It is suitable to detect GRIA2 in samples from Rat and Dog.

Quick Overview for GRIA2 antibody (AA 175-430) (ABIN199199)

Target: GRIA2 (Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2))

Reactivity: Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRIA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Radioimmunoassay (RIA)

Clone: 6C4

Product Details anti-GRIA2 Antibody

Binding Specificity: AA 175-430

Specificity: Recognizes rat Glutamate Receptor 2 (GluR2) at 102kD. Other proteins (66kD or lower MW) appear to be breakdown products of GluR2. In IHC, GluR2 is widely distributed at both the cellular and synaptic levels. Recognizes GluR2 present in a vast majority of, but not all, GABAergic interneurons. Does not cross-react with other AMPA/Kainate GluR subunits. Species cross-reactivity: Macaque monkey and canine.

Purification: Protein A purified

Immunogen: Recombinant fusion protein TrpE-GluR2 (putative N-terminal portion, aa175-430).

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, ICC, IHC, RIA, WB

Usage: Suitable for use in Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry and RIA. Immunohistochemistry (50um 4 % paraformaldehyde fixed tissue): 1:250-1:400. Immunocytochemistry: 4 % paraformaldehyde fixed cells. Positive control: Cerebral cortex, pyramidal neurons.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 40 % glycerol. No preservatives added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GRIA2

Target: GRIA2 (Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2))

Alternative Name: GRIA2 / GLUR2

Background: Name/Gene ID: GRIA2
Subfamily: Glutamate receptor - ionotropic (NMDA receptor)
Family: Ion Channel

Synonyms: GRIA2, AMPA 2, GluR-K2, Glutamate receptor 2, HBGR2, GluA2, GluR-2, GluR-B, GLUR2, GLURB

Gene ID: 2891

UniProt: P42262

Pathways: PI3K-Akt Signaling