ITGB1 antibody

Catalog No. ABIN199332

The Rat Monoclonal anti-ITGB1 antibody (Clone MB1-2) (ABIN199332) specifically detects ITGB1 in WB, FACS, IHC, IP, IHC (fro) and Func. The antibody is reactive with Mouse samples.

Quick Overview for ITGB1 antibody (ABIN199332)

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: MB1-2

Product Details anti-ITGB1 Antibody

Specificity: Reacts with b1 subunit of VLA (b1) integrins. Species cross-reactivity: mouse.

Purification: Protein A purified

Immunogen: Integrin, beta1.

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: Flo (10 μg/mL), Func, IHC, IHC-Fr (20 μg/mL), IP, WB (10 μg/mL)

Usage: Suitable for use in Immunoprecipitation, Flow Cytometry, Immunohistochemistry and Western Blot. Not suitable for use in Inhibition. Immunoprecipitation: 2 μg Lysate of 1E6 cell. Flow Cytometry: 10 μg/mL. Immunohistochemistry (Frozen): 20 μg/mL. As a result of the wide tissue distribution of VLA (b1), binding to diverse cell types is commonly observed. Western Blot: 10 μg/mL. Effective for samples that have been SDS-denatured and heated. Not effective for reduced samples. Inhibition: Does not block VLA integrin mediated cell adhesion to matrix proteins. Positive control: Human OVCAR5 cell line whole cell lysate.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.25 M sodium chloride, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: ITGB1 / Integrin Beta 1 / CD29

Background: Name/Gene ID: ITGB1
Family: Integrin

Synonyms: ITGB1, CD29, CD29 antigen, Integrin beta-1, MDF2, MSK12, Integrin VLA-4 beta subunit, VLA-BETA, VLAB, Beta 1 integrin, FNRB, GPIIA, Integrin Beta 1, VLA-4 subunit beta

Gene ID: 3688

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome