MAP2 antibody (AA 1-151)

Catalog No. ABIN199410

This anti-MAP2 antibody is a Mouse Monoclonal antibody detecting MAP2 in WB, IHC, IF and IHC (p). Suitable for Human, Rat, Cow and Xenopus laevis.

Quick Overview for MAP2 antibody (AA 1-151) (ABIN199410)

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reactivity: Human, Rat, Cow, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MAP2 Antibody

Binding Specificity: AA 1-151

Specificity: Highly specific to MAP2a, b, c and shows no cross-reactivity with other MAPs, tau and tubulin. Recognizes MAP2a and MAP2b (~280kD doublet) and MAP2c (70kD). Its epitope maps in the first 151 amino acids of MAP2. Reacts with dendrites and cell bodies of neurons. Species cross-reactivity: Human, bovine, rat, Xenopus and quail.

Purification: Protein G purified

Immunogen: Bovine brain microtubule associated protein 2. Epitope: aa1-151. Cellular Localization: Cytoplasmic.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: IF, IHC, IHC-P, WB (1 - 2 μg/mL)

Usage: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Western Blot: 1-2 μg/mL for 2hrs at RT. Immunohistochemistry (formalin/paraffin): 1-2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6 for 10-20 min., followed by cooling at RT for 20 min. Positive control: IMR-5 or T98G human glioblastoma cells or brain. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4.

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Alternative Name: MAP2 / MAP-2

Background: Name/Gene ID: MAP2

Synonyms: MAP2, MAP2C, MAP-2, MAP2A, MAP2B

Gene ID: 4133

UniProt: P11137