MAP2K7 antibody

Catalog No. ABIN199427

The Mouse Monoclonal anti-MAP2K7 antibody is suitable to detect MAP2K7 in samples from Human and Mouse. It has been validated for WB, ELISA and IF.

Quick Overview for MAP2K7 antibody (ABIN199427)

Target: MAP2K7 (Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAP2K7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-MAP2K7 Antibody

Specificity: Reacts with the ~47kD human and mouse MKK7 protein. There is no cross reactivity to MKK4. Reactivity is confirmed with MKK7 transfected COS cells, MDCK, mouse liver and Caco2 cell lysates. MDCK is the recommended positive control for this antibody.

Purification: Protein A purified

Immunogen: Recombinant GST-fusion protein containing full length human MKK7 sequence.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (15 - 20 μg/mL), WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunofluorescence, Western Blot and ELISA. Immunofluorescence: 15-20 μg/mL. Western Blot: 1-3 μg/mL. ELISA: 0.1-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for MAP2K7

Target: MAP2K7 (Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7))

Alternative Name: MAP2K7 / MEK7

Background: Name/Gene ID: MAP2K7
Subfamily: MAP2K
Family: Protein Kinase

Synonyms: MAP2K7, JNK kinase 2, MAP kinase kinase 7, MEK 7, Jnkk2, PRKMK7, SAPKK-4, SKK4, MAPK/ERK kinase 7, MAPKK 7, MAPKK7, SAPK kinase 4, JNK-activating kinase 2, JNKK 2, Map kinase kinase 7 beta 1, Map kinase kinase 7 gamma 2, MEK7, MKK7, SAPKK4

Gene ID: 5609

UniProt: O14733

Pathways: MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling