TGFB1 antibody

Catalog No. ABIN199718

The Mouse Monoclonal anti-TGFB1 antibody has been validated for WB, IHC, ELISA, Func and Neut. It is suitable to detect TGFB1 in samples from Human.

Quick Overview for TGFB1 antibody (ABIN199718)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGFB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Functional Studies (Func), Neutralization (Neut)

Product Details anti-TGFB1 Antibody

Specificity: Western Blot demonstrated reactivity to either dimeric (25kD) or monomeric (12.5kD) molecules of TGF-b under non-reducing and reducing condition, respectively. Recognizes both human platelet-derived and recombinant TGF-b in enzyme-linked in ELISA.

Purification: Protein G purified

Immunogen: TGF-b from human platelets.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Func, IHC, Neut, WB

Usage: Suitable for use in ELISA, Neutralization, Immunohistochemistry and Western Blot. Neutralization: Neutralizes TGF-b activity in vitro inhibition assay of CCL/64 cell growth and also neutralize the growth promoting action of TGF-b in the NRK-49F colony forming assays. The effect of microinjection of this MAb into one blastomere of two cell stage Xenopus embryos indicated that this antibody was able to neutralize effectively the bioactivity of TGF-b in vivo. Immunohistochemistry: Used to locate TGF-b within tissues.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.0, 40 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: TGFB1 / TGF Beta 1

Background: Name/Gene ID: TGFB1
Family: TGF beta

Synonyms: TGFB1, CED, DPD1, Diaphyseal dysplasia 1, LAP, Latency-associated peptide, TGF Beta, TGFbeta, TGF-beta 1 protein, TGF-beta-1, TGFB

Gene ID: 7040

UniProt: P01137

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints