TNF alpha antibody

Catalog No. ABIN199737

This anti-TNF alpha antibody is a Rabbit Polyclonal antibody detecting TNF alpha in WB, IHC, ELISA, FACS, IHC (p), Neut and IHC (fro). Suitable for Mouse and Rat.

Quick Overview for TNF alpha antibody (ABIN199737)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TNF alpha Antibody

Specificity: Recognizes both mouse natural and recombinant TNF-alpha and recognizes membrane and receptor bound TNF-alpha. Shows neutralizing activity. Species cross-reactivity: rat.

Purification: Purified

Immunogen: Mouse TNF-alpha.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo (1:10), IHC, IHC-Fr, IHC-P, Neut, WB (1:10)

Usage: Suitable for use in Immunohistochemistry, Flow Cytometry, ELISA, Western Blot and Neutralization. Immunohistochemistry (frozen and paraffin sections): 1:10. Flow Cytometry: 1:10. Western Blot: 1:10. Biological assays: Remove sodium azide and sterile filter.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % BSA

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF Alpha

Background: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Gene ID: 7124

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome