Caspase 1 antibody (AA 225-242)

Catalog No. ABIN200369

The Rabbit Polyclonal anti-Caspase 1 antibody has been validated for WB, IP and ICC. It is suitable to detect Caspase 1 in samples from Human, Mouse and Rat.

Quick Overview for Caspase 1 antibody (AA 225-242) (ABIN200369)

Target: Caspase 1 (CASP1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Caspase 1 Antibody

Binding Specificity: AA 225-242

Specificity: Recognizes human, mouse and rat caspase-1. Detects bands of ~45kD (procaspase-1) and ~20kD (cleavage product) by Western blot.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to aa 225-242 (KTSDSTFLVFMSHGIREG) of human caspase-1.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ICC (10 - 20 μg/mL), IP (4 - 8 μg/mL), WB (0.5 - 4 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 0.5-4 μg/mL. Immunocytochemistry:10-20 μg/mL. Immunoprecipitation: 4-8 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.5 % BSA, 0.01 % thimerosal, 30 % glycerol

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Caspase 1

Target: Caspase 1 (CASP1)

Alternative Name: CASP1 / Caspase 1

Background: Name/Gene ID: CASP1
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP1, Caspase-1, CASP-1, CASP1 nirs variant 1, Caspase 1, ICE, ICE-like protease, IL-1 beta-converting enzyme, IL-1BC, IL1BC, IL1BCE, p45, IL1B-convertase, Interleukin-1 beta convertase

Gene ID: 834

UniProt: P29466

Pathways: Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome