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Cyclin D1 antibody

CCND1 Reactivity: Human, Rat WB, ELISA, IHC, FACS, IP, IHC (p), IHC (fro) Host: Mouse Monoclonal unconjugated

Catalog No. ABIN200701

Datasheet as PDF

Target See all Cyclin D1 (CCND1) Antibodies

Cyclin D1 (CCND1)
Reactivity
210

115

109

40

20

18

16

12

9

8

3

3

3

3

3

2

2

1

1

1

1
Human, Rat
Host
178

54

1
Mouse
Compatible Secondaries
Clonality
166

65
Monoclonal
Conjugate
118

21

17

7

5

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1
This Cyclin D1 antibody is un-conjugated
Application
183

74

62

61

39

30

28

27

25

20

19

11

6

5

3

2

2

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity

Recognizes the Cyclin D1 (36kD). Species cross-reactivity: Human and rat.

Purification

Protein A purified

Immunogen

Human cyclin D1.

Type of Immunogen: Purified protein

Isotype

IgG1

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CCND1 Reactivity: Human, Mouse, Rat, Cow, Rabbit, Sheep WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Cyclin D1 (CCND1) (AA 68-97) antibody
CCND1 Reactivity: Human, Mouse WB, IF, IHC (p) Host: Rabbit Polyclonal RB8066 unconjugated

anti-Cyclin D1 (CCND1) (AA 1-295) antibody
CCND1 Reactivity: Human WB, ELISA, IHC, FACS Host: Mouse Monoclonal 1F7F5 unconjugated

anti-Cyclin D1 (CCND1) (AA 167-295) antibody
CCND1 Reactivity: Human ELISA, IHC, FACS, ICC Host: Mouse Monoclonal 3B6G3 unconjugated

anti-Cyclin D1 (CCND1) (C-Term) antibody
CCND1 Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated

anti-Cyclin D1 (CCND1) antibody
KO Validated CCND1 Reactivity: Human WB, IHC, IF, IP Host: Rabbit Monoclonal unconjugated

anti-Cyclin D1 (CCND1) (C-Term) antibody
CCND1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Cyclin D1 (CCND1) (AA 68-97) antibody
CCND1 Reactivity: Human, Mouse WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Cyclin D1 (CCND1) antibody
CCND1 Reactivity: Human, Mouse, Rat, Monkey WB, IHC, FACS, IF, StM Host: Mouse Monoclonal DCS-6 unconjugated

anti-Cyclin D1 (CCND1) (C-Term) antibody
CCND1 Reactivity: Human WB, IF, IP Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: ELISA, Flo, IHC, IHC-Fr, IHC-P, IP, WB (1:2000)

Usage: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen and paraffin), ELISA, Immunoprecipitation and Western Blot. Western Blot: 1:2000. Flow Cytometry: Neat, Use 5 μL of the optimal working dilution to stain 1 x10⁶ in 100 μL. Permeabilization is recommended prior to staining. Immunohistochemistry (Paraffin and frozen): 1:1000-1:10000. Note: Paraffin sections require antigen retrieval using sodium citrate buffer,  pH 6.0, heat treatment prior to staining. Positive control: Small intestine, colon.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

Tris-glycine, 0.1 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
Target

Cyclin D1 (CCND1)

Alternative Name

CCND1 / Cyclin D1 (CCND1 Products)

Background

Name/Gene ID: CCND1

Synonyms: CCND1, B-cell lymphoma 1 protein, BCL-1 oncogene, B-cell CLL/lymphoma 1, BCL-1, Cyclin D1, G1/S-specific cyclin-D1, U21B31, PRAD1, BCL1, D11S287E, PRAD1 oncogene

Gene ID

595

UniProt

P24385

Pathways

PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling