Cyclin D1 antibody

Catalog No. ABIN200701

This Mouse Monoclonal antibody specifically detects Cyclin D1 in WB, IHC, ELISA, FACS, IP, IHC (p) and IHC (fro). It exhibits reactivity toward Human and Rat.

Quick Overview for Cyclin D1 antibody (ABIN200701)

Target: Cyclin D1 (CCND1)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Cyclin D1 Antibody

Specificity: Recognizes the Cyclin D1 (36kD). Species cross-reactivity: Human and rat.

Purification: Protein A purified

Immunogen: Human cyclin D1.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo, IHC, IHC-Fr, IHC-P, IP, WB (1:2000)

Usage: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen and paraffin), ELISA, Immunoprecipitation and Western Blot. Western Blot: 1:2000. Flow Cytometry: Neat, Use 5 μL of the optimal working dilution to stain 1 x10⁶ in 100 μL. Permeabilization is recommended prior to staining. Immunohistochemistry (Paraffin and frozen): 1:1000-1:10000. Note: Paraffin sections require antigen retrieval using sodium citrate buffer,  pH 6.0, heat treatment prior to staining. Positive control: Small intestine, colon.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-glycine, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: CCND1 / Cyclin D1

Background: Name/Gene ID: CCND1

Synonyms: CCND1, B-cell lymphoma 1 protein, BCL-1 oncogene, B-cell CLL/lymphoma 1, BCL-1, Cyclin D1, G1/S-specific cyclin-D1, U21B31, PRAD1, BCL1, D11S287E, PRAD1 oncogene

Gene ID: 595

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling