Cyclin D1 antibody

Catalog No. ABIN200704

This Mouse Monoclonal antibody specifically detects Cyclin D1 in WB, IHC, ELISA, IP and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for Cyclin D1 antibody (ABIN200704)

Target: Cyclin D1 (CCND1)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cyclin D1 Antibody

Specificity: Recognizes cyclin D1. Does not cross-react with other cyclin proteins including other D type cyclins. Does not co-immunoprecipitate CDK4 kinase activity associated with cyclin D1. Species cross-reactivity: Human and mouse.

Purification: Protein A purified

Immunogen: Full length human cyclin D1 protein.

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P, IP, WB (0.3 - 1 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. ELISA: 0.1-1.0 μg/mL. Western Blot: 0.3-1.0 μg/mL. Immunoprecipitation: 5 μg. Immunohistochemistry: 2-10 μg/mL. Formalin-fixed paraffin embedded sections require a Heat Induced epitope Retrieval (HIER) step prior to staining.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: CCND1 / Cyclin D1

Background: Name/Gene ID: CCND1

Synonyms: CCND1, B-cell lymphoma 1 protein, BCL-1 oncogene, B-cell CLL/lymphoma 1, BCL-1, Cyclin D1, G1/S-specific cyclin-D1, U21B31, PRAD1, BCL1, D11S287E, PRAD1 oncogene

Gene ID: 595

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling