Fibronectin 1 antibody (Biotin)

Catalog No. ABIN200947

This Rabbit Polyclonal antibody specifically detects Fibronectin 1 in WB, ELISA, IHC, IHC (p) and IP. It exhibits reactivity toward Human.

Quick Overview for Fibronectin 1 antibody (Biotin) (ABIN200947)

Target: Fibronectin 1 (FN1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibronectin 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-Fibronectin 1 Antibody (Biotin)

Specificity: Recognizes human Fibronectin. Typically less than 1 % cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. Has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross-reactivity with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.

Purification: Immunoaffinity purified

Immunogen: Fibronectin purified from human plasma.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:4000 - 1:8000), IHC, IHC-P, IP, WB (1:5000 - 1:10000)

Usage: Suitable for use in ELISA, Immunoprecipitation, Immunohistochemistry and Western Blot. ELISA: 1:4000-1:8000. Assayed against 1 μg of Fibronectin in a standard capture ELISA using ABTS as a substrate for 30 minutes at RT. Immunohistochemistry (Paraffin embedded tissue): 1:50-1:200. Western Blot: 1:5000-1:10000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 100 μL sterile ddH20

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for Fibronectin 1

Target: Fibronectin 1 (FN1)

Alternative Name: FN1 / Fibronectin

Background: Name/Gene ID: FN1

Synonyms: FN1, CIG, Cold-insoluble globulin, Fibronectin 1, FINC, LETS, GFND, ED-B, Fibronectin, FN, FNZ, GFND2, Migration-stimulating factor, MSF

Gene ID: 2335

UniProt: P02751

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy