ITGAV/ITGB6 antibody

Catalog No. ABIN201125

The Mouse Monoclonal anti-ITGAV/ITGB6 antibody is suitable to detect ITGAV/ITGB6 in samples from Human, Mouse and Rat. It has been validated for FACS, IP and Func.

Quick Overview for ITGAV/ITGB6 antibody (ABIN201125)

Target: ITGAV/ITGB6 (Integrin alpha V, Integrin beta 6 (ITGAV/ITGB6))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGAV/ITGB6 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Product Details anti-ITGAV/ITGB6 Antibody

Specificity: Recognizes mouse integrin alpha V beta 6. Blocks activity against mouse and human alpha V beta 6. Does not recognize alpha V or other alpha V integrins. Species cross-reactivity: Human, rat and mouse. Because the antibody was raised in knockout mice, it is likely to recognize the receptor in other species.

Purification: Protein A purified

Immunogen: Immunization of beta 6 knockout mice with wild type murine keratinocytes.

Type of Immunogen: Cells

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo, Func, IP

Usage: Suitable for use in Flow Cytometry, Immunoprecipitation and Function Blocking. Not suitable for use in Western Blot and Immunohistochemistry. Flow Cytometry (effective for mouse and human): Use buffers containing 1 mM Mg, since the epitope recognized is divalent cation-dependent. Immunoprecipitation: Effective. Protein G or rabbit anti-mouse IgG recommended for capture. Function blocking: Inhibition of alpha V beta 6-mediated cell adhesion and cell migration are achieved at a concentration of 100 μg/mL using beta 6 transfected cells tested against TGFbeta LAP. Note: Cell lines adhere to simple ligands (e. g. fibronectin) using more integrins than alphav/beta6, so it would be necessary to also block other integrins (e. g. with anti-beta1 antibody) to see any effect of this antibody on adhesion to fibronectin. For example, H441 cells, which naturally express high levels of alphaV/beta6, may be used as a positive control.

Not recommended for: IHC, WB

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS. No preservatives added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ITGAV/ITGB6

Target: ITGAV/ITGB6 (Integrin alpha V, Integrin beta 6 (ITGAV/ITGB6))

Alternative Name: Integrin AlphaV Beta6