ITGAV/ITGB6 antibody

Catalog No. ABIN7863748

The Rat Monoclonal anti-ITGAV/ITGB6 antibody is suitable to detect ITGAV/ITGB6 in samples from Human. It has been validated for FACS, WB, ELISA and IF.

Quick Overview for ITGAV/ITGB6 antibody (ABIN7863748)

Target: ITGAV/ITGB6 (Integrin alpha V, Integrin beta 6 (ITGAV/ITGB6))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGAV/ITGB6 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: AvB6 53a-2

Product Details anti-ITGAV/ITGB6 Antibody

Purpose: Integrin alpha V + beta 6 Antibody

Specificity: Integrins are heterodimers composed of noncovalently associated transmembrane °™ and æ subunits. The 16 °™ and 8 æ subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, Collagen and Vitronectin. Certain integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors, both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Mouse fibroblasts retrovirally infected with cDNA to human beta6, Cells expressed a mouse/human avb6 chimera.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ITGAV/ITGB6

Target: ITGAV/ITGB6 (Integrin alpha V, Integrin beta 6 (ITGAV/ITGB6))

Alternative Name: Integrin alpha V + beta 6

Background:

antigen identified by monoclonal antibody L230, CD 51, CD51, Integrin alpha five, integrin alpha V beta 3, Integrin alpha-V light chain, integrin alphaVbeta3, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51), ITGAV, MSK 8, Msk8, Vitronectin receptor subunit alpha, VNRA, VTNR, ITGB6, Integrin, beta 6

Squamous cell carcinoma cells. A375M melanoma cell line. A549 cell line.

Molecular Weight: 150-160kDa under non-reducing conditions. Under reducing conditions|cleaved into 2 bands of ~125-130kDa +20-25kDa

Gene ID: 3685, 3694, 436873

UniProt: P06756, P18564