LEF1 antibody (AA 282-331)

Catalog No. ABIN202428

The Rabbit Polyclonal anti-LEF1 antibody (ABIN202428) specifically detects LEF1 in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat, Dog, Guinea Pig, Cow, Rabbit, Goat, Horse, Pig, Zebrafish (Danio rerio), Chicken, Monkey, Bat and Xenopus laevis samples.

Quick Overview for LEF1 antibody (AA 282-331) (ABIN202428)

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Cow, Rabbit, Goat, Horse, Pig, Zebrafish (Danio rerio), Chicken, Monkey, Bat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LEF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LEF1 Antibody

Binding Specificity: AA 282-331

Specificity: Human LEF1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa282-331 of human LEF1 (Q9UJU2, NP_057353). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB (2 μg/mL)

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Alternative Name: LEF1

Background: Name/Gene ID: LEF1

Synonyms: LEF1, LEF-1, TCF7L3, TCF1-alpha, TCF10, TCF1ALPHA

Gene ID: 51176

NCBI Accession: NP_057353

UniProt: Q9UJU2

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding