ITGB1 antibody

Catalog No. ABIN204312

Product Details anti-ITGB1 Antibody

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Specificity: Recognizes human Integrin beta 1. Reacts with calcium-dependent epitopes on the integrin b1 subunit. When bound to b1, activates b1 dependent binding to extra cellular matrix substrates. Species cross-reactivity: Rabbit and monkey.

Purification: Protein A purified

Immunogen: Human IL-1b activated endothelial cells.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo, ICC (2 μg/mL), IHC, IHC-Fr, IHC-P, IP

Usage: Suitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, FACS and ELISA. Immunocytochemistry: 2 μg/mL (use on numerous cell lines as long as Ca2+ is present),. Immunoprecipitation: 5 μg/400 μL lysate (0.5 % triton PBS plus Ca++). FACS: 2-5 μg/1 x10⁶, in the presence of Ca++. Stimulation of cells: 2-10 μg/mL per 1 x10⁶. Binding buffer must contain 1 mM Ca++. Immunohistochemistry: For use on acetone or mild paraformaldehyde fixed tissue (2-2.5 % PFA, short time) frozen and paraffin embedded (1:500, citrate HEIR treated, In et al 2003). Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.1. No preservatives.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Short-term: 4°C, Long-term: Add sterile 40-50% glycerol, aliquot, and store at -20°C.

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: ITGB1 / Integrin Beta 1 / CD29 (ITGB1 Products)

Background: Name/Gene ID: ITGB1
Family: Integrin

Synonyms: ITGB1, CD29, CD29 antigen, Integrin beta-1, MDF2, MSK12, Integrin VLA-4 beta subunit, VLA-BETA, VLAB, Beta 1 integrin, FNRB, GPIIA, Integrin Beta 1, VLA-4 subunit beta

Gene ID: 3688

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome