ITGB1 antibody

Catalog No. ABIN204312

The Mouse Monoclonal anti-ITGB1 antibody (ABIN204312) specifically detects ITGB1 in FACS, ELISA, IHC, IP, IHC (p), ICC and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for ITGB1 antibody (ABIN204312)

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ITGB1 Antibody

Specificity: Recognizes human Integrin beta 1. Reacts with calcium-dependent epitopes on the integrin b1 subunit. When bound to b1, activates b1 dependent binding to extra cellular matrix substrates. Species cross-reactivity: Rabbit and monkey.

Purification: Protein A purified

Immunogen: Human IL-1b activated endothelial cells.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo, ICC (2 μg/mL), IHC, IHC-Fr, IHC-P, IP

Usage: Suitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, FACS and ELISA. Immunocytochemistry: 2 μg/mL (use on numerous cell lines as long as Ca2+ is present),. Immunoprecipitation: 5 μg/400 μL lysate (0.5 % triton PBS plus Ca++). FACS: 2-5 μg/1 x10⁶, in the presence of Ca++. Stimulation of cells: 2-10 μg/mL per 1 x10⁶. Binding buffer must contain 1 mM Ca++. Immunohistochemistry: For use on acetone or mild paraformaldehyde fixed tissue (2-2.5 % PFA, short time) frozen and paraffin embedded (1:500, citrate HEIR treated, In et al 2003). Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.1. No preservatives.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Short-term: 4°C, Long-term: Add sterile 40-50% glycerol, aliquot, and store at -20°C.

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: ITGB1 / Integrin Beta 1 / CD29

Background: Name/Gene ID: ITGB1
Family: Integrin

Synonyms: ITGB1, CD29, CD29 antigen, Integrin beta-1, MDF2, MSK12, Integrin VLA-4 beta subunit, VLA-BETA, VLAB, Beta 1 integrin, FNRB, GPIIA, Integrin Beta 1, VLA-4 subunit beta

Gene ID: 3688

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome