MTOR antibody

Catalog No. ABIN204413

The Rat Monoclonal anti-MTOR antibody is suitable to detect MTOR in samples from Human and Mouse. It has been validated for ELISA and ICC.

Quick Overview for MTOR antibody (ABIN204413)

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This MTOR antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC)

Product Details anti-MTOR Antibody

Specificity: Recognizes human TOR. Species cross-reactivity: Mouse.

Purification: Protein G purified

Sterility: 0.2 μm filtered

Immunogen: E. coli-derived recombinant human TOR.

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, ICC (8 - 25 μg/mL)

Usage: Immunohistochemistry: This antibody was used at a concentration of 25 μg/mL with appropriate secondary reagents to detect TOR in paraformaldehyde-fixed HepG2 (human hepatocellular carcinoma) and MCF-7 (human breast adenocarcinoma) cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 200 μL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from 0.2 μm filtered PBS solution containing 5 % Trehalose

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MTOR

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Alternative Name: MTOR

Background: Name/Gene ID: MTOR
Subfamily: PI3/PI4
Family: Protein Kinase

Synonyms: MTOR, FRAP1, FRAP2, FRAP, Mammalian target of rapamycin, RAFT1, Rapamycin and FKBP12 target 1, RAPT1, Rapamycin target protein 1

Gene ID: 2475

UniProt: P42345

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect