R-Spondin 3 antibody

Catalog No. ABIN204684

This anti-R-Spondin 3 antibody is a Rat Monoclonal antibody detecting R-Spondin 3 in WB and ELISA. Suitable for Mouse.

Quick Overview for R-Spondin 3 antibody (ABIN204684)

Target: R-Spondin 3 (RSPO3)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This R-Spondin 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 400404

Product Details anti-R-Spondin 3 Antibody

Specificity: Selected for its ability to detect recombinant mouse RSPO-3 in direct ELISAs and Western Blots. In these formats, this antibody shows no cross-reactivity with rmRSPO-1, rmRSPO-4, rhRSPO-2, or rhRSPO-3.

Purification: Protein G purified

Immunogen: E. coli-derived rmRSPO-3.

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, WB

Usage: Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect mouse RSPO-3. Using a colorimetric detection system, the detection limit for rmRSPO-3 is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse RSPO-3. The detection limit for rmRSPO-3 is approximately 1 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS with 5 % trehalose

Handling Advice: Aviod frequent freeze thaws.

Storage: -20 °C

Storage Comment: Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.

Target Details for R-Spondin 3

Target: R-Spondin 3 (RSPO3)

Alternative Name: RSPO3

Background: Name/Gene ID: RSPO3

Synonyms: RSPO3, CRISTIN1, HRspo3, HPWTSR, R-spondin 3, Protein with TSP type-1 repeat, R-spondin 3 homolog, THSD2, PWTSR, R-spondin-3, Roof plate-specific spondin-3

Gene ID: 84870

UniProt: Q9BXY4