SOD2 antibody

Catalog No. ABIN204760

This Mouse Monoclonal antibody specifically detects SOD2 in WB, IHC, ICC and IHC (fro). It exhibits reactivity toward Human and Rat.

Quick Overview for SOD2 antibody (ABIN204760)

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SOD2 Antibody

Specificity: Recognizes human MnSOD. Species cross-reactivity: rat.

Purification: Protein G purified

Immunogen: Manganese superoxide dismutase (MnSOD).

Isotype: IgG1

Application Details

Application Notes: Approved: ICC, IHC, IHC-Fr (0.5 - 10 μg/mL), WB

Usage: Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Immunohistochemistry (Acetone fixed cryostat sections): 0.5-10 μg/mL using an APAAP or PAP procedure. Antibody also reacts with 4 % PFA fixed frozen tissue. Typical block contains 1 % non-fat dry milk or 3 % NGS and 0.2-1.5 % triton X-100 (higher triton is only used on thick sections, blocked for 1-3 hours at RT. Primary antibody is diluted only in PBS or TBS without excess blocking materials (no triton or milk), typical dilution is 1:200-1:1000 with incubations up to 48 hours at 4?C. Wash 3x10' in PBS and detect via DAB. Immunoreactive neurons are characterized by the dark brown, fine granular deposition in soma and proximal dendrites. Immunocytochemistry (cell smears): 0.5-10 μg/mL. Western Blot: Under reducing conditions a band at ~25kD will be identified.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for SOD2

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Alternative Name: MNSOD / SOD2

Background: Name/Gene ID: SOD2

Synonyms: SOD2, IPOB, MVCD6, Indophenoloxidase B, Mn superoxide dismutase, MNSOD, Mangano-superoxide dismutase

Gene ID: 6648

UniProt: P04179

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling