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Vimentin antibody

This Mouse Monoclonal antibody specifically detects Vimentin in WB, IHC, FACS, ICC and IHC (fro). It exhibits reactivity toward Human, Rat, Pig, Dog, Goat and Hamster.
Catalog No. ABIN204913

Quick Overview for Vimentin antibody (ABIN204913)

Target

See all Vimentin (VIM) Antibodies
Vimentin (VIM)

Reactivity

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Human, Rat, Pig, Dog, Goat, Hamster

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This Vimentin antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Specificity

    Reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e. g. lymphoma, sarcoma and melanoma. Species cross-reactivity: Human, rat, goat, canine, hamster and porcine.

    Purification

    Protein G purified

    Immunogen

    Vimentin.

    Type of Immunogen: Purified protein

    Isotype

    IgG1
  • Application Notes

    Approved: Flo (1:50 - 1:100), ICC, IHC, IHC-Fr, WB (1:50 - 1:500)

    Usage: Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Flow Cytometry: 1:50-1:100. Immunohistochemistry (frozen sections): 1:50-1:100. Avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Western Blot: 1:50-1:500. Optimum dilutions to be determined by researcher. Hybridoma: SP2/0-Ag14 mouse myeloma with spleen cells from a BALB/c mouse immunized with cytoskeletal vimentin extract of calf lens.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.2, 0.09 % sodium azide, 40 % glycerol. No stabilizing proteins added.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Target

    Vimentin (VIM)

    Alternative Name

    Vimentin

    Background

    Name/Gene ID: VIM

    Synonyms: VIM, Vimentin

    Gene ID

    7431

    UniProt

    P08670

    Pathways

    Caspase Cascade in Apoptosis
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