C5 antibody (N-Term)

Catalog No. ABIN206662

This Mouse Monoclonal antibody specifically detects C5 in WB, ELISA and Func. It exhibits reactivity toward Human.

Quick Overview for C5 antibody (N-Term) (ABIN206662)

Target: C5 (Complement Component 5 (C5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This C5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Functional Studies (Func)

Clone: 046-10-2-2 (10-04)

Product Details anti-C5 Antibody

Binding Specificity: N-Term

Specificity: Recognizes secreted complement component 5 (C5), a 190kD serine protease present in blood serum. It is composed of an 115kD disulphide linked alpha-chain and a 75 kD beta-chain. In addition to its role in complement, C5 is thought to be associated with inflammation, stimulating tumor necrosis factor alpha, interferon gamma mediated suppression of IL10 and IL12 production. C5 is activated via either the classical or the alternative complement pathway, by proteolytic cleavage of C5a from the N-terminal of the alpha-chain, by C5 convertase. C5a is an anaphylatoxin which mediates local inflammation. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. It also stimulates chemotaxis of neutrophils, eosinophils, basophils and monocytes towards the site of inflammation. The remaining fragment, C5b, initiates the assembly of the membrane attack complex. In hemolysis inhibition studies it was found that Will block C5 hemolytic activity. Removal of sodium azide is recommended prior to use in functional assays. XXX XXX recommend the use of EUQOO3 for this purpose.

Purification: Protein A purified

Immunogen: Purified human C5.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Borate buffered saline, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for C5

Target: C5 (Complement Component 5 (C5))

Alternative Name: Complement C5

Background: Name/Gene ID: C5

Synonyms: C5, Anaphylatoxin C5a analog, CPAMD4, Complement C5, Complement component 5

Gene ID: 727

UniProt: P01031

Pathways: Complement System, Carbohydrate Homeostasis