CD25 antibody
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- Target See all CD25 (IL2RA) Antibodies
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD25 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- Recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a 55kD type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts. XXX recommend the use of MCA273EL for this purpose.
- Purification
- Protein G purified
- Immunogen
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Stimulated Rat T cells.
Type of Immunogen: Cells - Clone
- OX-39
- Isotype
- IgG1
- Top Product
- Discover our top product IL2RA Primary Antibody
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anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 201-268) antibody
IL2RA Reactivity: Human, Mouse FACS, WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibodyIL2RA Reactivity: Human WB, IHC, ELISA, ICC Host: Mouse Monoclonal 1B5D12 unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) antibodyIL2RA Reactivity: Human WB, ELISA, IHC (fro), IHC (p), ICC Host: Rabbit Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibodyIL2RA Reactivity: Human, Mouse, Rat, Monkey WB, IHC, ELISA, IF/ICC Host: Mouse Monoclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) antibodyIL2RA Reactivity: Human FACS Host: Mouse Monoclonal MEM-181 unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-245) antibodyIL2RA Reactivity: Pig WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (PerCP)IL2RA Reactivity: Human FACS Host: Mouse Monoclonal MEM-181 PerCP
anti-Interleukin 2 Receptor, alpha (IL2RA) antibodyIL2RA Reactivity: Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 42-183) antibodyIL2RA Reactivity: Human IHC, StM Host: Mouse Monoclonal IL2RA-2395 unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-185) antibodyIL2RA Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Approved: ELISA, Flo (1:10 - 1:50), IHC, IHC-Fr, IHC-P, IP
Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. - Comment
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Target Species of Antibody: Rat
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, 0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- Alternative Name
- IL2RA / CD25 (IL2RA Products)
- Background
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Name/Gene ID: IL2RA
Family: Interleukin
Synonyms: IL2RA, CD25 antigen, CD25, IDDM10, IL-2 receptor subunit alpha, IL2R, Interleukin 2 receptor, alpha, IL-2-RA, IL2-RA, TCGFR, IL-2R subunit alpha, p55, TAC antigen - Gene ID
- 3559
- UniProt
- P01589
- Pathways
- JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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