CD25 antibody

Catalog No. ABIN206702

The Mouse Monoclonal anti-CD25 antibody has been validated for FACS, IHC, ELISA, IP, IHC (fro) and IHC (p). It is suitable to detect CD25 in samples from Rat.

Quick Overview for CD25 antibody (ABIN206702)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD25 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: OX-39

Product Details anti-CD25 Antibody

Specificity: Recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a 55kD type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts. XXX recommend the use of MCA273EL for this purpose.

Purification: Protein G purified

Immunogen: Stimulated Rat T cells.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo (1:10 - 1:50), IHC, IHC-Fr, IHC-P, IP

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: IL2RA / CD25

Background: Name/Gene ID: IL2RA
Family: Interleukin

Synonyms: IL2RA, CD25 antigen, CD25, IDDM10, IL-2 receptor subunit alpha, IL2R, Interleukin 2 receptor, alpha, IL-2-RA, IL2-RA, TCGFR, IL-2R subunit alpha, p55, TAC antigen

Gene ID: 3559

UniProt: P01589

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation