CD11b antibody

Catalog No. ABIN206753

The Mouse Monoclonal anti-CD11b antibody has been validated for FACS, IHC, IP and IHC (fro). It is suitable to detect CD11b in samples from Human, Baboon, Rhesus Monkey and Cynomolgus.

Quick Overview for CD11b antibody (ABIN206753)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human, Baboon, Rhesus Monkey, Cynomolgus

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CD11b Antibody

Specificity: Recognizes the human CD11b cell surface glycoprotein, a 165kD molecule also known as the alphaM integrin, MAC-1 and CR3. This molecule is expressed as a heterodimer in association with the beta 2 integrin, and is found upon monocytes, granulocytes, NK cells and some peripheral blood lymphocytes. Blocks some functions of CD11b, although full definition is still to be completed.

No Cross-Reactivity: Cat (Feline)

Purification: Protein G purified

Immunogen: Rheumatoid synovial cells and human monocytes.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:10 - 1:100), IHC, IHC-Fr (1:100 - 1:1000), IP

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: ITGAM / CD11b

Background: Name/Gene ID: ITGAM
Family: Integrin

Synonyms: ITGAM, Antigen CD11b (p170), CD11B, CD11b antigen, CR3A, Integrin alpha-M, MAC-1, MO1A, Neutrophil adherence receptor, MAC1A, SLEB6, CR-3 alpha chain

Gene ID: 3684

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation