CD4 antibody

Catalog No. ABIN206939

The Mouse Monoclonal anti-CD4 antibody has been validated for FACS, IP, Func, BR, Neut and Inhibition. It is suitable to detect CD4 in samples from Human.

Quick Overview for CD4 antibody (ABIN206939)

Target: CD4

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func), Blocking Reagent (BR), Neutralization (Neut), Blocking Antibody (Inhibition)

Clone: MEM-115

Product Details anti-CD4 Antibody

Specificity: Recognizes an epitope in the D1 domain of CD4 antigen, a 55 kD transmembrane glycoprotein expressed on a subset of T lymphocytes (helper T cells) and also on monocytes, tissue macrophages and granulocytes. It is negative in Western blotting even with non-reduced samples of cell lysates.

Purification: Protein A purified

Immunogen: Human thymocytes and T lymphocytes.

Type of Immunogen: Cells

Isotype: IgG2a

Application Details

Application Notes: Approved: Block, Flo, Func, Inhb, IP, Neut

Usage: Immunoprecipitation: excellent. Functional Application: The antibody MEM-115 blocks binding of HIV gp120 to CD4 Molecule and it also strongly inhibits CD4-MHC Class II interactions. Flow Cytometry: Recommended dilution - 3 μg/mL. Application notes:. Altho

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris buffered saline (TBS), 15 mM sodium azide, approx,  pH 8.0.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: Name/Gene ID: CD4

Synonyms: CD4, CD4 antigen, CD4 receptor, CD4mut, CD4 antigen (p55), CD4 Molecule

Gene ID: 920

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events