NEFM antibody

Catalog No. ABIN207002

The Mouse Monoclonal anti-NEFM antibody has been validated for WB, IHC, ICC, IHC (p) and BR. It is suitable to detect NEFM in samples from Pig.

Quick Overview for NEFM antibody (ABIN207002)

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Blocking Reagent (BR)

Clone: NF-09

Product Details anti-NEFM Antibody

Specificity: Reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kD) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

Purification: Protein A purified

Immunogen: Pellet of porcine brain cold stable proteins after depolymerization of microtubules.

Isotype: IgG2a

Application Details

Application Notes: Approved: Block, ICC, IHC, IHC-P, WB

Usage: Immunocytochemistry: Positive tissue - Neuro2A murine cell line. Application note - Carnoys fixative 2 x 3 min, blocking 1 % glycine + 0.2 % gelatin 10 min.

Comment:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for NEFM

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Alternative Name: NEFM / NF-M

Background: Name/Gene ID: NEFM

Synonyms: NEFM, 160 kDa neurofilament protein, NEF3, NF-M, NFM, Neurofilament 3

Gene ID: 4741

UniProt: P07197

Pathways: Brown Fat Cell Differentiation