Androgen Receptor antibody (pSer213)

Catalog No. ABIN207872

The Mouse Monoclonal anti-Androgen Receptor antibody has been validated for WB. It is suitable to detect Androgen Receptor in samples from Human and Monkey.

Quick Overview for Androgen Receptor antibody (pSer213) (ABIN207872)

Target: Androgen Receptor (AR)

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Androgen Receptor antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 156C135-2

Product Details anti-Androgen Receptor Antibody

Binding Specificity: AA 207-221, pSer213

Specificity: In IGF-1 stimulated LNCaP cells (passage number 38), a ~110 kD band was observed. Please see Lin et al. 2003 for additional details. The serine phosphorylation site recognized by this has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Baboon, Monkey, Marmoset (100%) Gorilla, Bat, Panda (93%) Dog, Bovine, Cat, Rabbit, Horse, Pig, Guinea pig (87%) Mouse, Rat, Hamster (80%).

Purification: Protein G purified

Immunogen: This antibody was developed against a synthetic peptide corresponding to amino acids 207-221 (GRAREAS*GAPTSSKD) of human androgen receptor, containing the serine 213 phosphorylation site: GenBank Accession No. A39248. Note: S* refers to phosphorylated serine in the peptide sequence. Percent identity by BLAST analysis: Human, Chimpanzee, Baboon, Monkey, Marmoset (100%), Gorilla, Bat, Panda (93%), Dog, Bovine, Cat, Rabbit, Horse, Pig, Guinea pig (87%), Mouse, Rat, Hamster (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (1 - 4 μg/mL)

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Short term: 4°C
Long term: -70°C.

Target Details for Androgen Receptor

Target: Androgen Receptor (AR)

Alternative Name: AR / Androgen Receptor

Background: Name/Gene ID: AR
Subfamily: NR3 Steroid receptor
Family: NHR

Synonyms: AR, Androgen receptor, Dihydrotestosterone receptor, DHTR, AIS, KD, NR3C4, HUMARA, SBMA, TFM, SMAX1, HYSP1

Gene ID: 367

UniProt: P10275

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding