Androgen Receptor antibody (N-Term)

Catalog No. ABIN185266

Product Details anti-Androgen Receptor Antibody

Target: Androgen Receptor (AR)

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Androgen Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)

Purpose: Androgen Receptor

Sequence: EVQLGLGRVY PRPPSC

Specificity: This antibody is expected to recognize isoform 1 (NP_000035.2) only.

Predicted Reactivity: Human, Mouse, Rat, Dog, Pig, Cow

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence EVQLGLGRVYPRPPSC, from the N Terminus of the protein sequence according to NP_000035.2.

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Approx 100 kDa band observed in Human Cerebellum lysates (calculated MW of 99.2 kDa according to NP_000035.2). Recommended concentration:0.1-0.5 μg/mL. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.

DS IHC Results: In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the mitochondria/cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.

Chromatin Immunoprecipitation: A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using EB06441 with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Androgen Receptor

Target: Androgen Receptor (AR)

Alternative Name: Androgen Receptor (AR Products)

Synonyms: AIS antibody, DHTR antibody, HUMARA antibody, HYSP1 antibody, KD antibody, NR3C4 antibody, SBMA antibody, SMAX1 antibody, TFM antibody, AW320017 antibody, Tfm antibody, Andr antibody, AR antibody, androgen receptor antibody, AR antibody, Ar antibody, ar antibody

Background: AR, androgen receptor, dihydrotestosterone receptor, KD, AIS, TFM, DHTR, SBMA, NR3C4, SMAX1, HUMARA, HYSP1, androgen receptor, dihydrotestosterone receptor, RP11-383C12.1

Gene ID: 367

NCBI Accession: NP_000035

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding