IRS1 antibody (pSer307)

Catalog No. ABIN208883

The Rabbit Polyclonal anti-IRS1 antibody is suitable to detect IRS1 in samples from Human, Mouse, Rat, Monkey, Rabbit, Dog and Horse. It has been validated for WB and ELISA.

Quick Overview for IRS1 antibody (pSer307) (ABIN208883)

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Reactivity: Human, Mouse, Rat, Monkey, Rabbit, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IRS1 Antibody

Binding Specificity: pSer307

Specificity: Recognizes human IRS-1, pS307. Minimal reactivity with non phosphorylated Ser307. Species sequence homology: mouse, rat, canine, vervet monkey 100 % , porcine, bovine -94 %, chicken 88 %.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to aa 298-316 of Human IRS-1 at 180kD. Peptide Sequence: GLTRRSRTE-pS-ITATSPASM.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:2000 - 1:4000), WB (1:250 - 1:1500)

Usage: Suitable for use in ELISA and Western Blot. ELISA: 1:2000-1:4000, Assayed against 0.1 μg of the immunizing peptide. Western Blot: 1:250-1:1500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide. No stabilizing proteins added.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Alternative Name: IRS1 / IRS-1

Background: Name/Gene ID: IRS1

Synonyms: IRS1, IRS-1, HIRS-1, Insulin receptor substrate 1

Gene ID: 3667

UniProt: P35568

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process