NF-kB p65 antibody (C-Term)

Catalog No. ABIN209099

The Rabbit Polyclonal anti-NF-kB p65 antibody is suitable to detect NF-kB p65 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IP.

Quick Overview for NF-kB p65 antibody (C-Term) (ABIN209099)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NF-kB p65 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-NF-kB p65 Antibody

Binding Specificity: C-Term

Specificity: Specific for the 65kD, DNA binding subunit of human NFkB (also referred to as Rel A). Reactivity with other endogenous proteins has not been detected. Based on sequenced homology, reactivity with NFkB from mouse and rat appears likely.

Purification: Immunoaffinity purified

Immunogen: A recombinant protein derived from the carboxyl terminal portion of the human NFkB protein.

Type of Immunogen: Recombinant protein

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC (0.5 - 2 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation. ELISA: 0.1-1 μg/mL. Western Blot: 1-3 μg/mL. Immunohistochemistry: 0.5-2 μg/mL. Immunoprecipitation: 2-5 μg/IP. Important: To achieve appropriate staining results, heat-induced epitope retrieval (Citrate buffer,  pH 6.0) is required.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: RELA / NFKB p65

Background: Name/Gene ID: RELA

Synonyms: RELA, NF-kappa-B p65delta3, NFKB3, p65, Transcription factor p65

Gene ID: 5970

UniProt: Q04206

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins