SGK1 antibody (AA 399-412)

Catalog No. ABIN210631

This Rabbit Polyclonal antibody specifically detects SGK1 in WB and IP. It exhibits reactivity toward Human.

Quick Overview for SGK1 antibody (AA 399-412) (ABIN210631)

Target: SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SGK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-SGK1 Antibody

Binding Specificity: AA 399-412

Specificity: Err:509

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%) Monkey, Marmoset (93%) Mouse, Rat, Opossum (86%).

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to amino acids 399-412 (C-GKSPDSVLVTASVK) of human SGK1, KLH-conjugated. Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey, Marmoset (93%), Mouse, Rat, Opossum (86%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg Immunoprecipitates SGK1 from 500 μg of Rat 2 lysate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SGK1

Target: SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Alternative Name: SGK1 / SGK

Background: Name/Gene ID: SGK1
Subfamily: SGK
Family: Protein Kinase

Synonyms: SGK1, Hsgk, Hsgk1, SGK, Sgk1 variant i3

Gene ID: 6446

UniProt: O00141

Pathways: MAPK Signaling, Notch Signaling, Steroid Hormone Mediated Signaling Pathway