SPRY2 antibody (AA 26-39)

Catalog No. ABIN210640

This Rabbit Polyclonal antibody specifically detects SPRY2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Pig and Hamster.

Quick Overview for SPRY2 antibody (AA 26-39) (ABIN210640)

Target: SPRY2 (Sprouty Homolog 2 (SPRY2))

Reactivity: Human, Mouse, Rat, Dog, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPRY2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SPRY2 Antibody

Binding Specificity: AA 26-39

Specificity: Recognizes Sprouty2, Mr ~35-40kD. A non-specific protein was also detected, Mr ~70kD. Species cross-reactivity: Human and mouse. Cross-reactivity with rat predicted, based on sequence homology.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Panda, Dog, Pig, Opossum (100%) Marmoset, Bovine, Horse (93%) Elephant, Rabbit, Turkey, Chicken, Platypus (86%).

Purification: Protein A purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to aa26-39 (C-GEPDPRDALTQQVH) of human Sprouty2, with an N-terminal cysteine added for conjugation purposes. The immunizing sequence is identical in rat and mouse. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Panda, Dog, Pig, Opossum (100%), Marmoset, Bovine, Horse (93%), Elephant, Rabbit, Turkey, Chicken, Platypus (86%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.1 - 1 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot: 0.1-1 μg/mL detects Sprouty2 in RIPA lysates from HeLa and 3T3/A31 cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SPRY2

Target: SPRY2 (Sprouty Homolog 2 (SPRY2))

Alternative Name: SPRY2 / Sprouty 2

Background: Name/Gene ID: SPRY2

Synonyms: SPRY2, HSPRY2, Protein sprouty homolog 2, Sprouty homolog 2 (Drosophila), Sprouty 2, Spry-2, Sprouty (Drosophila) homolog 2

Gene ID: 10253

UniProt: O43597

Pathways: EGFR Signaling Pathway, Sensory Perception of Sound, EGFR Downregulation