SPRY2 antibody (N-Term)

Catalog No. ABIN2784217

There is 1 publication for this product available. The Rabbit Polyclonal anti-SPRY2 antibody is suitable to detect SPRY2 in samples from Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Pig and Guinea Pig. It has been validated for WB and IHC.

Quick Overview for SPRY2 antibody (N-Term) (ABIN2784217)

Target: SPRY2 (Sprouty Homolog 2 (SPRY2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Pig, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPRY2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SPRY2 Antibody

Binding Specificity: N-Term

Purpose: SPRY2 Antibody - N-terminal region

Sequence: MEARAQSGNG SQPLLQTPRD GGRQRGEPDP RDALTQQVHV LSLDQIRAIR

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 92%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SPRY2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SPRY2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8112758

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-SPRY2 antibody (ABIN2784217)

Hsu, Lee, Hartstein, Harocopos: "Clostridium perfringens Keratitis Leading to Blinding Panophthalmitis." in: Cornea, Vol. 27, Issue 10, pp. 1200-3, (2008) (PubMed).

Target Details for SPRY2

Target: SPRY2 (Sprouty Homolog 2 (SPRY2))

Alternative Name: SPRY2

Background:

Background Information: SPRY2 is a protein belonging to the sprouty family. It contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. SPRY2 is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. SPRY2 may play a role in alveoli branching during lung development as shown by a similar mouse protein.This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Sprouty homolog 2 (Drosophila)

Alternative Symbols: IGAN3, hSPRY2

Protein Name: Protein sprouty homolog 2

Molecular Weight: 35kDa

Gene ID: 10253

NCBI Accession: NP_005833

UniProt: O43597

Pathways: EGFR Signaling Pathway, Sensory Perception of Sound, EGFR Downregulation