STAT5B antibody (AA 774-787)

Catalog No. ABIN210645

This anti-STAT5B antibody is a Rabbit Polyclonal antibody detecting STAT5B in WB and IP. Suitable for Human, Mouse, Rat, Cow, Dog, Horse, Pig and Hamster.

Quick Overview for STAT5B antibody (AA 774-787) (ABIN210645)

Target: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT5B antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-STAT5B Antibody

Binding Specificity: AA 774-787

Specificity: Recognizes the 90-92kD forms of STAT5B protein. Species cross-reactivity: Human and mouse.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%) Panda, Bat, Chicken, Xenopus (93%) Opossum (86%).

Purification: Protein A purified

Immunogen: Amino acids 774-787 of human STAT5B (GRPMDSQWIPHAQS). The peptide was coupled to KLH prior to immunization. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%), Panda, Bat, Chicken, Xenopus (93%), Opossum (86%).

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB

Usage: Western Blot: 0.5-2 mg/mL detects STAT5B (doublet of 90kD and 92kD) in. human A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure. Immunoprecipitation: 4 μg immunoprecipitates STAT5B from A431 carcinoma cells and a. previous lot immunoprecipitated STAT5B from human Raji cells. A RIPA lysate of human A431 cells (500 μg protein) was incubated with 4 μg of anti-STAT 5B Immune complexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B. Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection. Positive Antigen Control: mouse 3T3 cell lysate. Add 2.5 μL of 2-mercaptoethanol. per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for STAT5B

Target: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Alternative Name: STAT5B / STAT

Background: Name/Gene ID: STAT5B

Synonyms: STAT5B, Transcription factor STAT5B, STAT5

Gene ID: 6777

UniProt: P51692

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation