STAT5B antibody (N-Term)

Catalog No. ABIN6744443

This Rabbit Polyclonal antibody specifically detects STAT5B in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Horse, Pig, Rabbit and Chicken.

Quick Overview for STAT5B antibody (N-Term) (ABIN6744443)

Target: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Reactivity: Human, Mouse, Rat, Dog, Horse, Pig, Rabbit, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT5B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-STAT5B Antibody

Binding Specificity: N-Term

Specificity: Human STAT5b

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Rabbit, Horse, Pig, Chicken (100%) Bovine (91%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human STAT5B (P51692, NP_036580). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Galago, Mouse, Rat, Elephant, Panda, Dog, Rabbit, Horse, Pig, Opossum, Zebra finch, Chicken (100%), Bovine, Bat, Guinea pig, Xenopus (92%), Monkey, Sheep, Hamster, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for STAT5B

Target: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Alternative Name: STAT5B / STAT

Background: Name/Gene ID: STAT5B

Synonyms: STAT5B, Transcription factor STAT5B, STAT5

Gene ID: 6777

NCBI Accession: NP_036580

UniProt: P51692

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation