Cyclin E1 antibody

Catalog No. ABIN210870

This anti-Cyclin E1 antibody is a Mouse Monoclonal antibody detecting Cyclin E1 in WB, ELISA, IP and ICC. Suitable for Human.

Quick Overview for Cyclin E1 antibody (ABIN210870)

Target: Cyclin E1 (CCNE1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin E1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Cyclin E1 Antibody

Specificity: Recognizes human p52 cyclin E. Cross-reactivity with mouse and rat was not observed.

Purification: Protein G purified

Immunogen: Recombinant human Cyclin E.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC (10 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunocytochemistry. Proteins were visualized by using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg immunoprecipitated cyclin E from 500 μg of human A431 RIPA cell lysate as confirmed by Western blot analysis of the immunoprecipitate using rabbit polyclonal anti-cyclin E. Immunocytochemistry: 10 μg/mL detected cyclin E in 50 % ethanol/50 % acetic acid-fixed human A431 cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: CCNE1 / Cyclin E1

Background: Name/Gene ID: CCNE1

Synonyms: CCNE1, Cyclin E1, CCNE, Cyclin Es, Cyclin Et, G1/S-specific cyclin-E1, G1/s-specific cyclin e, Cyclin E

Gene ID: 898

UniProt: P24864

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases