E2F4 antibody

Catalog No. ABIN210875

The Mouse Monoclonal anti-E2F4 antibody is suitable to detect E2F4 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IP.

Quick Overview for E2F4 antibody (ABIN210875)

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This E2F4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-E2F4 Antibody

Specificity: Recognizes E2F-4 at 55kD. Species cross-reactivity: Human, mouse and rat.

Purification: Protein G purified

Immunogen: Fusion Protein corresponding to the human E2F-4 protein.

Type of Immunogen: Fusion protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 0.5-2 μg/mL detected E2F4 in RIPA lysates from non-stimulated A431 cell lysate. Previous lots have detected E2F4 in HeLa nuclear extract, mouse 3T3 fibroblasts, and rat L6 skeletal myoblasts. Immunoprecipitation: 4-10 μg immunoprecipitated E2F4 from 500 μg of mouse 3T3/A431 cell lysate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine buffer,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for E2F4

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Alternative Name: E2F4

Background: Name/Gene ID: E2F4

Synonyms: E2F4, E2F-4, p107/p130-binding protein, Transcription factor E2F4

Gene ID: 1874

UniProt: Q16254

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size