JNK antibody (pThr183, pThr221, pTyr185, pTyr223)

Catalog No. ABIN210904

This Rabbit Polyclonal antibody specifically detects JNK in WB and IP. It exhibits reactivity toward Human, Mouse, Rat, Chicken, C. elegans and Drosophila melanogaster.

Quick Overview for JNK antibody (pThr183, pThr221, pTyr185, pTyr223) (ABIN210904)

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reactivity: Human, Mouse, Rat, Chicken, C. elegans, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JNK antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-JNK Antibody

Binding Specificity: AA 181-190, AA 219-229, pThr183, pThr221, pTyr185, pTyr223

Specificity: Recognizes phosphorylated JNK, Mr 46kD and 55kD. Species cross-reactivity: Human, rat, mouse. Expected to cross-react with Drosophila, C. elegans and chicken based on sequence homology.

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to aa181-190 [(MM[pT]P[pY]VVTRY-C)] of human/mouse/rat JNK1 (MAPK 8) and human/mouse/rat JNK2 (MAPK 9). Sequence also matches aa219-229 of human/mouse/rat JNK3.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IP (1 μg/mL), WB (1 μg/mL)

Usage: Suitable for use in Immunoprecipitation/Western Blot. Immunoprecipitation/Western Blot: 1 μg/mL. Anti-pan JNK/SAPK1 was used to Immunoprecipitate JNK from lysates of ultraviolet light-treated RAW 264 macrophage. Phosphorylated JNK (T183/Y185) was detected. Phosphorylated JNK was not detected in non-treated lysates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, before the addition of glycerol to 30 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Alternative Name: MAPK8 / JNK1 / JNK

Background: Name/Gene ID: MAPK8
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK8, JNK, JNK1, JNK1A2, JNK-46, MAP kinase 8, PRKM8, SAPK1, SAPK1c, MAPK 8, C-Jun N-terminal kinase 1, JNK21B1/2, JUN N-terminal kinase

Gene ID: 5599

UniProt: P45983

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins