Nibrin antibody (AA 420-774)

Catalog No. ABIN210930

This anti-Nibrin antibody is a Mouse Monoclonal antibody detecting Nibrin in WB and IP. Suitable for Human.

Quick Overview for Nibrin antibody (AA 420-774) (ABIN210930)

Target: Nibrin (NBN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nibrin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-Nibrin Antibody

Binding Specificity: AA 420-774

Specificity: Recognizes human Nibrin. Recognizes Nibrin/Nbs1, Mr 95kD. A non-specific protein was also detected at Mr 33kD in some lysates, but not Jurkat cell lysate. Species cross-reactivity: Human, NOT mouse.

Purification: Protein G purified

Immunogen: GST fusion protein corresponding to residues 420-774 of human Nibrin.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 2 μg/mL detects Nibrin/Nbs1 in Jurkat, HeLa nuclear extract, HeLa whole cell, and A431+/-EGF lysates. Included Positive Antigen Control: Jurkat lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. Immunoprecipitation: 4 μg immunoprecipitates Nibrin/Nbs1 from 500 μg of Jurkat RIPA lysate. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti- Nibrin/Nbs1 (2 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Nibrin/Nbs1 (95kD).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 100 mM Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Nibrin

Target: Nibrin (NBN)

Alternative Name: NBN / NBS1 / Nibrin

Background: Name/Gene ID: NBN

Synonyms: NBN, ATV, AT-V2, Nibrin, NBS1, AT-V1, NBS, p95

Gene ID: 4683

UniProt: O60934

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response