Cyclin A antibody (AA 417-432)

Catalog No. ABIN211135

This Rabbit Polyclonal antibody specifically detects Cyclin A in WB, IP, ICC and Func. It exhibits reactivity toward Human.

Quick Overview for Cyclin A antibody (AA 417-432) (ABIN211135)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin A antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Functional Studies (Func)

Product Details anti-Cyclin A Antibody

Binding Specificity: AA 417-432

Specificity: Recognizes human p58 cyclin A. Does not cross-react with cyclin B or cyclin D. Species cross-reactivity: Mouse, rat and sheep.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (93%) Hamster, Elephant, Panda, Dog, Bovine (87%) Rat, Horse, Platypus (80%).

Purification: Protein G purified

Immunogen: Synthetic peptide (KLH-coupled) corresponding to aa 417-432 (C-KHGVSLLNPPETLNL) within the C-terminus of human p58 cyclin A. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (93%), Hamster, Elephant, Panda, Dog, Bovine (87%), Rat, Horse, Platypus (80%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: Func, ICC (10 μg/mL), IP (1 - 5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Proteins were visualized using goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 1-5 μg/mL. The precipitate may be used to perform a histone H1 kinase assay. Immunocytochemistry: 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Alternative Name: CCNA2 / Cyclin A2

Background: Name/Gene ID: CCNA2

Synonyms: CCNA2, Cyclin-A2, Cyclin A2, CCN1, CCNA, Cyclin-A, G2/mitotic-specific cyclin a

Gene ID: 890

UniProt: P20248

Pathways: PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA