Cyclin E1 antibody (C-Term)

Catalog No. ABIN211139

The Mouse Monoclonal anti-Cyclin E1 antibody has been validated for WB, IP and GS. It is suitable to detect Cyclin E1 in samples from Human.

Quick Overview for Cyclin E1 antibody (C-Term) (ABIN211139)

Target: Cyclin E1 (CCNE1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Gel Shift (GS)

Clone: HE-172

Product Details anti-Cyclin E1 Antibody

Binding Specificity: C-Term

Specificity: Recognizes human cyclin E. The epitope is located within the last 80aa of the C-terminal. Detects a ~50kD band on human HeLa cell lysates by Western Blot analysis.

Purification: Protein A purified

Immunogen: Full-length, recombinant, human cyclin E protein.

Type of Immunogen: Recombinant protein

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: GS, IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Gel Mobility Shift Assay. Western Blot: 1-2 μg/mL. Immunoprecipitation: 1 μg/IP.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C, Long term: aliquot to Avoid freeze-thaw cycles, and store at -20°C.

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: CCNE1 / Cyclin E1

Background: Name/Gene ID: CCNE1

Synonyms: CCNE1, Cyclin E1, CCNE, Cyclin Es, Cyclin Et, G1/S-specific cyclin-E1, G1/s-specific cyclin e, Cyclin E

Gene ID: 898

UniProt: P24864

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases